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rabbit anti human pstat5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti human pstat5
    Rabbit Anti Human Pstat5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+pstat5/Phospho-Stat5+(Tyr694)+XP+Rabbit+mAb/10__1158_slash_2767___9764__crc___24___0286-85-9-12
    Average 96 stars, based on 335 article reviews
    rabbit anti human pstat5 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Activation of Cell Intrinsic Signaling in CAR-T cells via a Chimeric IL7R Domain
    Article Snippet: Total protein was quantified using a BCA protein quantification kit. (Pierce) Electrophoresis was conducted using Novex WedgeWell 10% Bis-Tris Mini Gels (ThermoFisher Scientific) and protein was transferred to polyvinylidene difluoride (PVDF) membranes. .. Western blot analysis was performed with the following antibodies: rabbit anti-human pSTAT5 (Cell Signaling Technology), rabbit anti-human STAT5 (Cell Signaling Technology), and mouse antihuman GAPDH (Invitrogen). .. For detection of STAT5 phosphorylation using TR-FRET, STAT5 (Total) and STAT5 (Phospho-Tyr 694/699) TR-FRET assay kits (Cayman Chemicals) were used.



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    CD123-targeted CAR and CCR can be coexpressed with maintained functionality. A, Schematic showing CAR and CCR structures. B, Transduction efficiency measured using flow cytometric staining of Halo and SNAP tags. n = 3 to 6 unique T-cell donors, unless noted no significant difference between groups. C, Measurement of <t>phosphorylated</t> <t>STAT5</t> percentage in cytokine starved, engineered T cells measured with and without activation on the immobilized rhCD123 target. n = 3 to 4 unique T-cell donors, comparison of CCR + CAR + and CAR + to NT (black) and with stimulation (colored). D, Soluble IL2 and IFNγ measured in the supernatant following coculture of unmodified (NT), CCR + , CAR + , or CCR + CAR + T cells with CD123-negative (K562) and CD123-positive (K562.CD123, MV-4-11, and Molm-13) targets. n = 3 to 6 unique T-cell donors; data represented as mean ± SD. Significance noted is in comparison to NT (black asterisks) and/or CCR + cells (green asterisks) or as noted. NT vs. CCR + comparison was nonsignificant in all instances. E, Bioluminescence-based cytotoxicity assays performed using K562, K562.CD123, MV-4-11, and Molm-13 stably expressing ffLuc; n = 3 to 5 donors. For B–E, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Unless noted, comparisons were nonsignificant. NT, nontransduced.
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    CD123-targeted CAR and CCR can be coexpressed with maintained functionality. A, Schematic showing CAR and CCR structures. B, Transduction efficiency measured using flow cytometric staining of Halo and SNAP tags. n = 3 to 6 unique T-cell donors, unless noted no significant difference between groups. C, Measurement of phosphorylated STAT5 percentage in cytokine starved, engineered T cells measured with and without activation on the immobilized rhCD123 target. n = 3 to 4 unique T-cell donors, comparison of CCR + CAR + and CAR + to NT (black) and with stimulation (colored). D, Soluble IL2 and IFNγ measured in the supernatant following coculture of unmodified (NT), CCR + , CAR + , or CCR + CAR + T cells with CD123-negative (K562) and CD123-positive (K562.CD123, MV-4-11, and Molm-13) targets. n = 3 to 6 unique T-cell donors; data represented as mean ± SD. Significance noted is in comparison to NT (black asterisks) and/or CCR + cells (green asterisks) or as noted. NT vs. CCR + comparison was nonsignificant in all instances. E, Bioluminescence-based cytotoxicity assays performed using K562, K562.CD123, MV-4-11, and Molm-13 stably expressing ffLuc; n = 3 to 5 donors. For B–E, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Unless noted, comparisons were nonsignificant. NT, nontransduced.

    Journal: Cancer Research Communications

    Article Title: Activation of Cell-Intrinsic Signaling in CAR-T Cells via a Chimeric IL7R Domain

    doi: 10.1158/2767-9764.CRC-24-0286

    Figure Lengend Snippet: CD123-targeted CAR and CCR can be coexpressed with maintained functionality. A, Schematic showing CAR and CCR structures. B, Transduction efficiency measured using flow cytometric staining of Halo and SNAP tags. n = 3 to 6 unique T-cell donors, unless noted no significant difference between groups. C, Measurement of phosphorylated STAT5 percentage in cytokine starved, engineered T cells measured with and without activation on the immobilized rhCD123 target. n = 3 to 4 unique T-cell donors, comparison of CCR + CAR + and CAR + to NT (black) and with stimulation (colored). D, Soluble IL2 and IFNγ measured in the supernatant following coculture of unmodified (NT), CCR + , CAR + , or CCR + CAR + T cells with CD123-negative (K562) and CD123-positive (K562.CD123, MV-4-11, and Molm-13) targets. n = 3 to 6 unique T-cell donors; data represented as mean ± SD. Significance noted is in comparison to NT (black asterisks) and/or CCR + cells (green asterisks) or as noted. NT vs. CCR + comparison was nonsignificant in all instances. E, Bioluminescence-based cytotoxicity assays performed using K562, K562.CD123, MV-4-11, and Molm-13 stably expressing ffLuc; n = 3 to 5 donors. For B–E, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Unless noted, comparisons were nonsignificant. NT, nontransduced.

    Article Snippet: Western blot analysis was performed with the following antibodies: rabbit anti–human pSTAT5 (Cell Signaling Technology), rabbit anti–human STAT5 (Cell Signaling Technology), and mouse anti–human GAPDH (Invitrogen).

    Techniques: Transduction, Staining, Activation Assay, Comparison, Stable Transfection, Expressing

    Hinge modifications do not improve antigen specificity of CD123-targeted chimeric IL7Rα. A, Amino acid sequences of hinge regions used in functional studies. B, Transduction efficiency measured using his-tagged rhCD123 binding of engineered receptors and flow cytometry. n = 3 unique T-cell donors. C, Quantification of phosphorylated STAT5 percentage in CCR-T cells measured with and without activation on the immobilized rhCD123 target. n = 3 unique T-cell donors, comparison between pSTAT5% in cytokine-starved cells with or without rhCD123 activation; *, P < 0.05. D, Representative Western blot testing IgG4 vs. IH STAT5 activation with and without immobilized rhCD123. NT, nontransduced.

    Journal: Cancer Research Communications

    Article Title: Activation of Cell-Intrinsic Signaling in CAR-T Cells via a Chimeric IL7R Domain

    doi: 10.1158/2767-9764.CRC-24-0286

    Figure Lengend Snippet: Hinge modifications do not improve antigen specificity of CD123-targeted chimeric IL7Rα. A, Amino acid sequences of hinge regions used in functional studies. B, Transduction efficiency measured using his-tagged rhCD123 binding of engineered receptors and flow cytometry. n = 3 unique T-cell donors. C, Quantification of phosphorylated STAT5 percentage in CCR-T cells measured with and without activation on the immobilized rhCD123 target. n = 3 unique T-cell donors, comparison between pSTAT5% in cytokine-starved cells with or without rhCD123 activation; *, P < 0.05. D, Representative Western blot testing IgG4 vs. IH STAT5 activation with and without immobilized rhCD123. NT, nontransduced.

    Article Snippet: Western blot analysis was performed with the following antibodies: rabbit anti–human pSTAT5 (Cell Signaling Technology), rabbit anti–human STAT5 (Cell Signaling Technology), and mouse anti–human GAPDH (Invitrogen).

    Techniques: Functional Assay, Transduction, Binding Assay, Flow Cytometry, Activation Assay, Comparison, Western Blot